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 หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Lipid Peroxidation and Antioxidant Activity in Molting Stages of Mud Crab (Scylla serrata)) ผู้เขียน: ดร.จินตนา สและน้อย, รองศาสตราจารย์ ,  Pakawan Setthamongkol, ภัทราวดี ศรีมีเทียน, ดร.เกรียงไกร สถาพรวานิชย์, ผู้ช่วยศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractLipid peroxidation (LPO) is the process whereby oxygen free radicals steal electrons from polyunsaturated fatty acids of cell membranes, initiating a self-propagating chain reaction and the end-products of the reactions are dangerous to the viability of cells. It is used as an indicator of oxidative stress or the amount of free radicals in cells and tissues. The observation of LPO activities in various organs of mud crab (Scylla serrata) over the molting cycle showed evidence that supported the functions of free radicals. LPO in the intermolt stage was significantly (P < 0.05) lower than at the premolt stage. The LPO activities gradually increased when the crab entered the premolt stage which was detected in the haemolymph, gill, hepatopancreas, integument and muscle of crabs. The hepatopancreas seemed to be the organ where the free radical processes occurred. LPO in the haemolymph, integument and gills had the highest levels 2 d before molting while the activities in the hepatopancreas showed distinguishing functions 2 wk before molting. The determination of antioxidant activity using free radical scavengers showed the same pattern in the gills, meat, hepatopancreas and claws. The activities gradually decreased from the intermolt stage until they reached a minimum in the early postmolt, and then increased again at late postmolt. It clearly indicated that LPO activities in the molting cycle corresponded with the physiology during the stage changing and adaptation of crab. |
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หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Variations of Catalase and Glutathione Activities in Molting Cycle of Mud Crab (Scylla serrata)) ผู้เขียน:ดร.จินตนา สและน้อย, รองศาสตราจารย์, ภัทราวดี ศรีมีเทียน, ดร.มิ่งขวัญ มิ่งเมือง, รองศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractThe activities of the antioxidative enzyme, catalase (CAT), and the non-enzyme, glutathione (GSH) in 12 stages over the molting of mud crab (Scylla serrata) collected from Chanthaburi Province were determined. The stages were defined as: A1 (6-hour postmolt), A2.1 (12-hour postmolt), A2.2 (24-hour postmolt), B1 (2-day postmolt), B 2.1 (3-day postmolt), B2.2 ( 5-day postmolt), B2.3 (7-day postmolt), B2.4 (10 day postmolt), C (intermolt), D1 (2-week premolt), D2 (1- week premolt) and D3 (2-day premolt). The results revealed that CAT which controlled and eliminated excessive H2O2, increased in premolt (D1–D3) then dropped in the early postmolt stages (A1–A2.2) and increased again in the late postmolt stages (B1–B2.4). GSH, a reducing substance, which is also involved in the detoxification of H2O2 showed the same pattern in most tissues except in the hepatopancreas where it increased from the early postmolt stage (A1) and continued to increase through the postmolt stages. The variation in activity of CAT and GSH corresponded well with the metabolic activity through the molt cycle. |
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